|
KMID : 0191119980130020165
|
|
Journal of Korean Medical Science
1998 Volume.13 No. 2 p.165 ~ p.170
|
|
|
Chemokines, osteopontin, ICAM-1 gene expression in cultured rat mesangial cells
|
|
Lee SK
Park JY/Chung SJ/Yang WS/Kim SB/Park SK/Park JS
|
|
|
Abstract
|
|
|
|
To investigate whether MCP-1, CINC, RANTES, osteopontin and ICAM-1 mRNA could be induced in cultured rat mesangial cells by interleukin-1β(IL-1beta), tumor necrosis factor-α (TNF-α) and lipopolysaccharide (LPS), and whether MCP-1 and CINC gene expression could be modulated by dexamethasone, Northern blot assays were performed. IL-1β induced MCP-1, CINC, RANTES and ICAM-1 gene expression in a time dependent manner. IL-1β-induced MCP-1, CINC and ICAM-1 mRNA amount were maximal at 3 hours exposure around 14.5, 15.7, 2.2 folds increase and IL-1β-induced RANTES mRNA at 24 hours around 2.0 folds. TNF-α and LPS also induced MCP-1 and ICAM-1 gene expression. TNF-α also induced RANTES gene expression but LPS did not. On the other hand, IL-1β, TNF-α and LPS had little effect on osteopontin gene expression but fetal calf serum could increase osteopontin mRNA. Dexamethasone suppressed the IL-1β-induced MCP-1 and CINC mRNA. These results suggest that, through these gene expressions, mesangial cells are able to communicate directly or indirectly with macrophages or neutrophils, which may lead to glomerulosclerosis.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
    
|
|